recipe duolink mounting medium (Bio-Rad)
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recipe duolink mounting medium
Recipe Duolink Mounting Medium, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recipe+duolink+mounting+medium/Mounting+Medium/pmc11795341-39-118-136
Average 93 stars, based on 165 article reviews
Recipe Duolink Mounting Medium, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recipe+duolink+mounting+medium/Mounting+Medium/pmc11795341-39-118-136
Average 93 stars, based on 165 article reviews
recipe duolink mounting medium - by Bioz Stars,
2026-09
93/100 stars
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Paraffin Section:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Saline:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Permeability:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see In Situ:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Proximity Ligation Assay:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Ligation:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Amplification:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Staining:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Blocking Assay:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Fluorescence:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Microscopy:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Modification:Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells Article Snippet: .. Cells, mice, and/or paraffin‐section tissue Fixation solution (see recipe) Phosphate‐buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes 100% ethanol ddH 2 O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma‐Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma‐Aldrich, cat. no. DUO92008) PLA kit (Sigma‐Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma‐Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see Article Title: Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells. Article Snippet: Cells, mice, and/or paraffin-section tissue Fixation solution (see recipe) Phosphate-buffered saline (PBS), pH 7.4 (Thermo Scientific, cat. no. 10010023) Optimal cutting temperature (OCT) medium Xylenes Crabtree et al. 3 of 16 Current Protocols 26911299, 2025, 2, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70043 by N at Prov Indonesia, W iley O nline L ibrary on [12/02/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 100% ethanol ddH2O 0.2% permeability solution (see recipe) Duolink kits: In situ red starter kit mouse/rabbit (Sigma-Aldrich, cat. no. DUO92101) In situ detection reagents red subkit (Sigma-Aldrich, cat. no. DUO92008) PLA kit (Sigma-Aldrich, cat. no. DUO92008) In situ wash buffers (Sigma-Aldrich, cat. no. DUO82049) Mfn1 monoclonal antibody (Abcam, cat. no. ab126575) Mfn2 monoclonal antibody (Abcam, cat. no. ab124773) PLA probes (see recipe) Wash buffer A (see recipe) 1× ligation buffer, 1:5 dilution (see recipe) Working ligase buffer (see recipe) Amplification buffer, 1:5 dilution (see recipe) Working amplifaction solution, 1:80 dilution (see |